Identification of CpG oligodeoxynucleotide sequences that induce IFN-gamma production in canine peripheral blood mononuclear cells

Vet Immunol Immunopathol. 2004 Dec 28;102(4):441-50. doi: 10.1016/j.vetimm.2004.08.004.

Abstract

Oligodeoxynucleotides containing the cytosine-phosphate-guanine (CpG) motif (CpG-ODNs) have been shown to induce T(H)1 immune responses in animals. Since the sequences of CpG-ODNs that induce T(H)1 responses are considered to vary among animal species, it is necessary to identify effective CpG-ODNs in each animal. In order to identify the sequences of CpG-ODNs that induce T(H)1 responses in dogs, mRNA expression and protein production of IFN-gamma were examined in peripheral blood mononuclear cells (PBMCs) from healthy dogs treated with 11 kinds of synthetic CpG-ODNs. One of the 11 CpG-ODNs (No. 2 CpG-ODN, 5'-GGTGCATCGATGCAGGGGGG-3') was shown to significantly increase mRNA expression and protein production of IFN-gamma in canine PBMCs in a manner dependent on the sequence of the CpG motif. This CpG-ODN also enhanced the expression of IL-12 p40 mRNA in canine PBMCs, whereas expression of IL-12 p35, IL-18, and IL-4 mRNAs was not induced by this CpG-ODN. These results indicate that this CpG-ODN was able to produce IFN-gamma by induction of T(H)1-skewed immune response in dogs. CpG-ODNs may be useful for inducing prophylactic and therapeutic immunity against allergic diseases, viral infection, and tumors in dogs.

Publication types

  • Clinical Trial
  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Dinucleoside Phosphates / immunology*
  • Dogs / immunology*
  • Female
  • Gene Expression Regulation / immunology
  • Interferon-gamma / biosynthesis*
  • Interleukin-12 / biosynthesis
  • Interleukin-18 / biosynthesis
  • Interleukin-4 / biosynthesis
  • Leukocytes, Mononuclear / immunology*
  • Male
  • Oligodeoxyribonucleotides / immunology*
  • RNA, Messenger / metabolism

Substances

  • Dinucleoside Phosphates
  • Interleukin-18
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Interleukin-12
  • Interleukin-4
  • cytidylyl-3'-5'-guanosine
  • Interferon-gamma