Recruitment of coat proteins onto Golgi membranes in intact and permeabilized cells: effects of brefeldin A and G protein activators

Cell. 1992 Apr 3;69(1):129-38. doi: 10.1016/0092-8674(92)90124-u.

Abstract

Brefeldin A (BFA) causes a rapid redistribution of coat proteins (e.g., gamma-adaptin) associated with the clathrin-coated vesicles that bud from the trans-Golgi network (TGN), while the clathrin-coated vesicles that bud from the plasma membrane are unaffected. gamma-Adaptin redistributes with the same kinetics as beta-COP, a coat protein associated with the non-clathrin-coated vesicles that bud from the Golgi complex. Upon removal of BFA, however, gamma-adaptin recovers its perinuclear distribution more rapidly. Redistribution of both proteins can be prevented by pretreating cells with AlF4-. Recruitment of adaptors from the cytosol onto the TGN membrane has been reconstituted in a permeabilized cell system and is increased by addition of GTP gamma S and blocked by addition of BFA. These results suggest a role for G proteins in the control of the clathrin-coated vesicle cycle at the TGN and further extend the similarities between clathrin-coated vesicles and non-clathrin-coated vesicles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Protein Complex alpha Subunits
  • Adaptor Protein Complex gamma Subunits
  • Adaptor Proteins, Vesicular Transport
  • Aluminum / pharmacology
  • Aluminum Compounds*
  • Blotting, Western
  • Brefeldin A
  • Cell Line
  • Coatomer Protein
  • Cyclopentanes / pharmacology*
  • Fluorides*
  • Fluorine / pharmacology
  • GTP-Binding Proteins / physiology
  • Golgi Apparatus / drug effects
  • Golgi Apparatus / metabolism*
  • Guanosine 5'-O-(3-Thiotriphosphate) / analogs & derivatives
  • Guanosine 5'-O-(3-Thiotriphosphate) / pharmacology
  • Microscopy, Fluorescence
  • Microtubule-Associated Proteins / metabolism*
  • Proteins / metabolism*

Substances

  • Adaptor Protein Complex alpha Subunits
  • Adaptor Protein Complex gamma Subunits
  • Adaptor Proteins, Vesicular Transport
  • Aluminum Compounds
  • Coatomer Protein
  • Cyclopentanes
  • Microtubule-Associated Proteins
  • Proteins
  • guanosine 5'-O-(1-thiotriphosphate)
  • Brefeldin A
  • tetrafluoroaluminate
  • Fluorine
  • Guanosine 5'-O-(3-Thiotriphosphate)
  • Aluminum
  • GTP-Binding Proteins
  • Fluorides