Abstract
Aim:
To construct phage display library of anti-chymopapain scFv.
Methods:
V(H) and V(L) gene repertoires were amplified from splenocyte mRNA by RT-PCR and joined by a (Gly(4)ser)3 linker to obtain scFv genes. The scFv genes were then cloned into phagemid pFAB5C to construct phage display library. Affinity selection and ELISA were used to identify specific phage antibody to chymopapain.
Results:
After 4 rounds of panning, high affinity scFv was obtained.
Conclusion:
Phage display library of anti-chymopapain scFv was successfully constructed, and scFv with binding ability to chymopapain was obtained.
Publication types
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English Abstract
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Chymopapain / immunology*
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Chymopapain / metabolism
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Female
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Genes, Immunoglobulin Heavy Chain*
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Genes, Immunoglobulin Light Chain*
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Immunoglobulin Heavy Chains / genetics
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Immunoglobulin Heavy Chains / metabolism
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Immunoglobulin Light Chains / genetics
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Immunoglobulin Light Chains / metabolism
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Immunoglobulin Variable Region / genetics*
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Immunoglobulin Variable Region / metabolism
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Mice
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Mice, Inbred BALB C
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Peptide Library*
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Protein Binding
Substances
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Immunoglobulin Heavy Chains
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Immunoglobulin Light Chains
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Immunoglobulin Variable Region
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Peptide Library
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Chymopapain