Abstract
ATG genes are required for autophagy-related processes that transport proteins/organelles destined for proteolytic degradation to the vacuole. Here, we describe the identification and characterisation of the Hansenula polymorpha ATG21 gene. Its gene product Hp-Atg21p, fused to eGFP, had a dual location in the cytosol and in peri-vacuolar dots. We demonstrate that Hp-Atg21p is essential for two separate modes of peroxisome degradation, namely glucose-induced macropexophagy and nitrogen limitation-induced microautophagy. In atg21 cells subjected to macropexophagy conditions, sequestration of peroxisomes tagged for degradation is initiated but fails to complete.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Autophagy*
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Autophagy-Related Proteins
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Cytosol / metabolism
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Fungal Proteins / genetics
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Fungal Proteins / metabolism*
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Genes, Fungal
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Glucose / metabolism
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Green Fluorescent Proteins / metabolism
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Microscopy, Fluorescence
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Mutation
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Peroxisomes / metabolism
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Peroxisomes / ultrastructure
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Pichia / genetics
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Pichia / growth & development
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Pichia / metabolism*
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Pichia / ultrastructure
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Recombinant Fusion Proteins / metabolism
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Saccharomyces cerevisiae Proteins / metabolism*
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Vacuoles / metabolism
Substances
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Atg23 protein, S cerevisiae
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Autophagy-Related Proteins
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Fungal Proteins
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Recombinant Fusion Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Glucose