Enzyme immuno assays for simultaneous screening of antibodies to HIV-1 and HIV-2 (EIA HIV-1 + HIV-2) have recently been developed. Confirming all reactive EIA HIV-1/2 screening results by Western blot (WB) for HIV-1 and HIV-2 antibodies is expensive. Six different EIA HIV-1/2 screening assays and one supplemental Line immuno assay (INNO-LIA HIV-1/HIV-2 Ab (LIA)) for confirmation of reactive EIA HIV-1/2 screening assay results were carried out on a panel of 400 sera of which 13% were HIV-1- and 2.5% were HIV-2-positive. The LIA was used as the 'gold standard'. Retrospectively, the results of the six EIA HIV-1/2 were evaluated in pairs (A and B), applying B to those sera reactive in A. A+B+ results were reported as positive. A+B- were either interpreted as negative or the LIA result of A+B- was accepted as the final result. At least seven of the EIA HIV-1/2 pairs gave rise to no false-positive or -negative results. This strategy was 5 times faster and resulted in a budget on average 50% lower than that of the conventional strategy. Further investigation of these alternative confirmatory strategies, in which the proposed algorithms are applied in sequential use of the different screening assays, are needed under field conditions in developing countries.