Abstract
Three new phloroglucinols, garcinielliptones K (1), L (2), and M (3), and two new terpenoids, garcinielliptones N (4) and O (5), have been isolated from the seeds of Garcinia subelliptica. The structures of 1-5 including their relative configurations were elucidated by spectroscopic methods and supported by computer-generated molecular modeling. Compounds 2 and 3 showed potent inhibitory effects on the release of beta-glucuronidase, and on beta-glucuronidase and histamine, respectively, from peritoneal mast cells stimulated with p-methoxy-N-methylphenethylamine (compound 48/80) in a concentration-dependent manner. Compounds 2 and 3 showed potent effects on NO production in culture media of RAW 264.7 cells in response to lipopolysaccharide (LPS). Compound 2 also showed a potent effect on NO production in culture media of N9 cells in response to LPS/interferon-gamma (IFN-gamma).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Inflammatory Agents, Non-Steroidal / chemistry
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Anti-Inflammatory Agents, Non-Steroidal / isolation & purification*
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Anti-Inflammatory Agents, Non-Steroidal / pharmacology
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Cells, Cultured
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Garcinia / chemistry*
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Interferon-gamma / metabolism
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Lipopolysaccharides / metabolism
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Mast Cells / drug effects
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Mice
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Molecular Structure
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Neutrophils / drug effects
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Nitric Oxide / antagonists & inhibitors
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Nitric Oxide / biosynthesis
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Nuclear Magnetic Resonance, Biomolecular
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Phloroglucinol / chemistry
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Phloroglucinol / isolation & purification*
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Phloroglucinol / pharmacology
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Plants, Medicinal
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Taiwan
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Terpenes / chemistry
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Terpenes / isolation & purification*
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Terpenes / pharmacology
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Triterpenes / chemistry
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Triterpenes / isolation & purification*
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Triterpenes / pharmacology
Substances
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Anti-Inflammatory Agents, Non-Steroidal
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Lipopolysaccharides
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Terpenes
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Triterpenes
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garcinielliptone
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Nitric Oxide
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Interferon-gamma
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Phloroglucinol