Enzymatic syntheses and selective hydrolysis of O-beta-D-galactopyranosides using a marine mollusc beta-galactosidase

Bioorg Med Chem Lett. 2005 Jan 3;15(1):139-43. doi: 10.1016/j.bmcl.2004.10.016.

Abstract

The use of crude extract of the hepatopancreas of Aplysia fasciata, a large mollusc belonging to the order Anaspidea containing a beta-galactosidase activity, was reported for the synthesis of different galactosides. Good yields with polar acceptors and the uncommon beta-1-3 selectivity in the transgalactosylation reactions with most of the acceptors were observed. A beta-1-2 selectivity in the hydrolytic conditions was also observed and discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aplysia / enzymology*
  • Galactose / metabolism*
  • Hydrolysis
  • beta-Galactosidase / metabolism*

Substances

  • beta-Galactosidase
  • Galactose