Abstract
Diseases associated with Escherichia coli infection are the subject of renewed interest due to emerging conditions such as hemolytic uremia syndrome. A collection of 15 strains of beta-hemolytic E. coli was isolated from diarrheic feces and diseased tissues of ferrets. All 15 strains were positive in specific PCR assays for the presence of hlyA, pap1, and cnf1. Seven of the cnf1-positive isolates were tested and shown to have a cytopathic effect on HeLa cell monolayers. The pathogenesis of these strains warrants future study.
MeSH terms
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Animals
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Bacterial Toxins / genetics*
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Bacterial Toxins / metabolism
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Diarrhea
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Escherichia coli / genetics
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Escherichia coli / pathogenicity*
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Escherichia coli Infections / microbiology
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Escherichia coli Infections / veterinary*
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Escherichia coli Proteins / genetics*
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Escherichia coli Proteins / metabolism
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Female
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Ferrets / microbiology*
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Genotype
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HeLa Cells
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Hemolysin Proteins / metabolism
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Humans
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Pancreatitis-Associated Proteins
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Serotyping
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Virulence Factors / genetics*
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Virulence Factors / metabolism
Substances
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Bacterial Toxins
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Escherichia coli Proteins
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Hemolysin Proteins
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Pancreatitis-Associated Proteins
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REG3A protein, human
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Virulence Factors
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cytotoxic necrotizing factor type 1