Butter and walnuts, but not olive oil, elicit postprandial activation of nuclear transcription factor kappaB in peripheral blood mononuclear cells from healthy men

Am J Clin Nutr. 2004 Dec;80(6):1487-91. doi: 10.1093/ajcn/80.6.1487.

Abstract

Background: Nuclear transcription factor kappaB (NF-kappaB) plays an important role in atherosclerosis by modulating gene expression. Postprandial lipemia has been correlated with an increase in NF-kappaB activation in vascular cells and it is associated with an increase in postprandial triacylglycerol-rich lipoproteins, which are involved in the development of atherosclerotic plaque.

Objective: The objective of this study was to determine the effect of the intakes of 3 different foods with different fat compositions on the postprandial activation of monocyte NF-kappaB.

Design: Eight healthy men followed a 4-wk baseline diet and then consumed 3 fat-load meals consisting of 1 g fat/kg body wt (65% fat) according to a randomized crossover design. Each meal had a different fatty acid composition, and the consumption of each meal was separated by 1 wk. The compositions of the 3 test meals were as follows: olive oil meal [22% saturated fatty acids (SFAs), 38% monounsaturated fatty acids (MUFAs), 4% polyunsaturated fatty acids (PUFAs), and 0.7% alpha-linolenic acid], butter meal (38% SFAs, 22% MUFAs, 4% PUFAs, and 0.7% alpha-linolenic acid), and walnut meal (20% SFAs, 24% MUFAs, 16% PUFAs, and 4% alpha-linolenic acid).

Results: Ingestion of the olive oil meal did not elicit NF-kappaB activation compared with ingestion of either the butter meal at 3 h (P <0.05) or the walnut meal at 9 h (P <0.05). There was no significant difference in the postprandial triacylglycerol response between the 3 meals.

Conclusions: Consumption of an olive oil-enriched meal does not activate NF-kappaB in monocytes as do butter and walnut-enriched meals. This effect could enhance the cardioprotective effect of olive oil-enriched diets.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arteriosclerosis / metabolism
  • Arteriosclerosis / prevention & control
  • Butter*
  • Cross-Over Studies
  • Dietary Fats / administration & dosage*
  • Fatty Acids / administration & dosage
  • Fatty Acids, Monounsaturated / administration & dosage
  • Fatty Acids, Unsaturated / administration & dosage
  • Gene Expression Regulation
  • Humans
  • Intercellular Adhesion Molecule-1 / blood
  • Juglans*
  • Leukocytes, Mononuclear / metabolism*
  • Lipids / blood
  • Male
  • NF-kappa B / blood
  • NF-kappa B / metabolism*
  • Olive Oil
  • Plant Oils
  • Postprandial Period*
  • Triglycerides / blood
  • Triglycerides / chemistry

Substances

  • Dietary Fats
  • Fatty Acids
  • Fatty Acids, Monounsaturated
  • Fatty Acids, Unsaturated
  • Lipids
  • NF-kappa B
  • Olive Oil
  • Plant Oils
  • Triglycerides
  • Intercellular Adhesion Molecule-1
  • Butter