Abstract
Macrophages represent one of the primary targets of HIV-1 infection. Changes in gene expression in primary human monocyte-derived macrophages following virus exposure were assessed using oligonucleotide arrays. Over a third of the 100 most modulated genes belonged to the interferon system. Upregulated interferon-stimulated genes included those essential for the innate immune response and also those involved in interferon and virus signal transduction from the cell surface. The promoter regions of a cluster of highly upregulated interferon-stimulated genes were analyzed for common regulatory elements. The nuclear factor in activated T cells (NFAT) and members of the interferon family of transcription factors appeared to be responsible for the upregulation of this set of interferon-stimulated genes following HIV-1 exposure.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Cells, Cultured
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism
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Gene Expression Profiling
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HIV Infections / virology
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HIV-1 / pathogenicity*
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Humans
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Interferons / genetics
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Interferons / metabolism*
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Interferons / pharmacology
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Macrophage Activation
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Macrophages / immunology
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Macrophages / virology*
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Monocytes / immunology
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Monocytes / virology
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NFATC Transcription Factors
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Oligonucleotide Array Sequence Analysis*
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Proteins / genetics
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Proteins / metabolism*
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Transcription Factors / genetics
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Transcription Factors / metabolism
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Up-Regulation*
Substances
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DNA-Binding Proteins
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NFATC Transcription Factors
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Nuclear Proteins
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Proteins
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Transcription Factors
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Interferons