Crystallization and preliminary X-ray diffraction analysis of suramin, a highly charged polysulfonated napthylurea, complexed with a myotoxic PLA2 from Bothrops asper venom

Biochim Biophys Acta. 2004 Dec 1;1703(1):83-5. doi: 10.1016/j.bbapap.2004.08.009.

Abstract

Suramin is a highly charged polysulfonated napthylurea that interferes in a number of physiologically relevant processes such as myotoxicity, blood coagulation and several kinds of cancers. This synthetic compound was complexed with a myotoxic Lys49 PLA(2) from Bothrops asper venom and crystallized by the hanging-drop vapor diffusion method at 18 degrees C. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell parameters a=49.05, b=63.84 and c=85.67 angstroms. Diffraction data was collected to 1.78 angstroms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Bothrops*
  • Crotalid Venoms / chemistry*
  • Crotalid Venoms / enzymology
  • Crystallography, X-Ray
  • Group II Phospholipases A2
  • Lysine / chemistry
  • Molecular Structure
  • Phospholipases A / chemistry
  • Phospholipases A / isolation & purification
  • Phospholipases A / metabolism*
  • Phospholipases A / toxicity*
  • Phospholipases A2
  • Suramin / analysis*
  • Suramin / chemistry
  • Suramin / metabolism
  • Urea / analogs & derivatives*
  • Urea / metabolism*
  • X-Ray Diffraction

Substances

  • Crotalid Venoms
  • Suramin
  • Urea
  • Phospholipases A
  • Group II Phospholipases A2
  • Phospholipases A2
  • Lysine