Abstract
Retroviral infection triggers the cytoplasmic translocation of two Crm1-dependent shuttle factors, namely the Ini1 (integrase interactor 1, hSNF5) and the promyelocytic leukemia (PML) protein. Blocking nuclear export of shuttle factors by leptomycin B increases the efficiency of retroviral integration, suggesting that some may mediate antiviral activity. While PML was shown to counteract proviral establishment, it remained unclear whether Ini1, a protein implicated in various processes during human immunodeficiency virus replication, has the same potential. Employing RNA interference-mediated knock-down of Ini1, we show here that the simultaneous accumulation of both proteins in the cytoplasm likely reflects two non-interdependent phenomena. Furthermore, Ini1 does not interfere with retroviral integration, as cells lacking Ini1 show no increased infection susceptibility.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Annexin A5 / metabolism
-
Antibiotics, Antineoplastic / pharmacology
-
Blotting, Western
-
Cell Fractionation
-
Chromosomal Proteins, Non-Histone
-
Cytoplasm / metabolism*
-
DNA-Binding Proteins / drug effects
-
DNA-Binding Proteins / metabolism*
-
Fatty Acids, Unsaturated / pharmacology
-
Fluorescent Antibody Technique
-
HeLa Cells
-
Humans
-
Neoplasm Proteins / biosynthesis*
-
Neoplasm Proteins / drug effects
-
Nuclear Proteins / biosynthesis*
-
Nuclear Proteins / drug effects
-
Polymerase Chain Reaction
-
Promyelocytic Leukemia Protein
-
RNA Interference
-
Retroviridae Infections / enzymology*
-
Retroviridae Infections / metabolism
-
SMARCB1 Protein
-
Transcription Factors / biosynthesis*
-
Transcription Factors / drug effects
-
Tumor Suppressor Proteins
-
Virus Integration / physiology*
Substances
-
Annexin A5
-
Antibiotics, Antineoplastic
-
Chromosomal Proteins, Non-Histone
-
DNA-Binding Proteins
-
Fatty Acids, Unsaturated
-
Neoplasm Proteins
-
Nuclear Proteins
-
Promyelocytic Leukemia Protein
-
SMARCB1 Protein
-
SMARCB1 protein, human
-
Transcription Factors
-
Tumor Suppressor Proteins
-
PML protein, human
-
leptomycin B