Cod106, a novel synaptic protein expressed in sensory hair cells of the inner ear and in CNS neurons

Ellen Reisinger; Ulrike Zimmermann; Marlies Knipper; Jost Ludwig; Nikolaj Klöcker; Bernd Fakler; Dominik Oliver

doi:10.1016/j.mcn.2004.08.018

Cod106, a novel synaptic protein expressed in sensory hair cells of the inner ear and in CNS neurons

Mol Cell Neurosci. 2005 Jan;28(1):106-17. doi: 10.1016/j.mcn.2004.08.018.

Authors

Ellen Reisinger¹, Ulrike Zimmermann, Marlies Knipper, Jost Ludwig, Nikolaj Klöcker, Bernd Fakler, Dominik Oliver

Affiliation

¹ Department of Physiology II, University of Freiburg, 79104 Freiburg, Germany.

PMID: 15607946
DOI: 10.1016/j.mcn.2004.08.018

Abstract

The exquisite performance of the highly specialized mammalian inner ear requires a multitude of specific proteins. Yet, only a subset of these proteins has been identified and studied in detail. Here, we describe a novel gene expressed in the organ of Corti that encodes a membrane-associated protein of 106 kDa. The new protein, termed Cod106, lacks sequence homology to characterized gene products. As shown by in situ hybridization, it is expressed in auditory and vestibular hair cells, as well as in distinct sets of CNS neurons with particularly high abundance in hippocampus and cerebellum. Immunohistochemistry detected Cod106 at the basal, synaptic pole of cochlear outer hair cells and vestibular hair cells. In cultured hippocampal neurons, Cod106 immunofluorescence co-localized with the postsynaptic density protein 95 (PSD95), indicating a postsynaptic localization. Cell-type specificity and subcellular localization may be consistent with an involvement of Cod106 in synaptic processes.

MeSH terms

Amino Acid Sequence
Animals
Animals, Newborn
Base Sequence
Brain / cytology
Brain / metabolism*
Cells, Cultured
Cerebellum / metabolism
Cochlea / cytology
Cochlea / metabolism*
DNA, Complementary / analysis
DNA, Complementary / genetics
Fluorescent Antibody Technique
Green Fluorescent Proteins
Hair Cells, Auditory / cytology
Hair Cells, Auditory / metabolism*
Hippocampus / metabolism
Intracellular Membranes / metabolism
Membrane Proteins / genetics
Membrane Proteins / isolation & purification
Membrane Proteins / metabolism*
Molecular Sequence Data
Nerve Tissue Proteins / genetics
Nerve Tissue Proteins / isolation & purification
Nerve Tissue Proteins / metabolism*
Rats
Synaptic Membranes / metabolism*
Synaptic Transmission / physiology
Vestibule, Labyrinth / cytology
Vestibule, Labyrinth / metabolism

Substances

DNA, Complementary
Membrane Proteins
Mycbpap protein, rat
Nerve Tissue Proteins
postsynaptic density proteins
Green Fluorescent Proteins

Associated data

GENBANK/AY353958
GENBANK/AY353959