Automated assays to study longevity in C. elegans

Mech Ageing Dev. 2005 Jan;126(1):139-45. doi: 10.1016/j.mad.2004.09.010.

Abstract

The nematode Caenorhabditis elegans is excellently suited as a model for studying the genetic and molecular genetic basis of aging, and to test chemical compounds that interfere with the aging process. Mutants of factors in both the insulin and target of rapamycin (TOR) signalling pathways have been shown to extend life span of the worm. Phenotypic similarities among those mutants suggested that, exploiting the corresponding phenotypes in a semiautomated way, may increase the speed of investigating life span and aging in C. elegans. Here, we discuss several methodological approaches to automate longevity assays in the nematode.

MeSH terms

  • Aging / genetics*
  • Aging / physiology
  • Animals
  • Automation / methods
  • Caenorhabditis elegans
  • Gene Expression Regulation / genetics*
  • Genes, Helminth*
  • Models, Genetic*
  • Signal Transduction / genetics*
  • Signal Transduction / physiology