Structural characterization of the dihydroflavonol 4-reductase B (DFR-B) gene in the sweet potato

DNA Seq. 2004 Aug;15(4):277-82. doi: 10.1080/10425170400004120.

Abstract

A genomic fragment containing the dihydroflavonol 4-reductase B (DFR-B) gene was cloned from the sweet potato (Ipomoea batatas) and its nucleotide sequence was analyzed. The exons and flanking regions were highly homologous to those of previously reported DFR-B genes of the Japanese morning glory, whereas the introns and the intergenic region were less conserved. In addition to the sequences of three miniature inverted-repeat transposable elements (MITEs) and one direct repeat previously reported in the DFR-B gene of Japanese morning glory, two mobile element-like sequences were newly identified in the sweet potato DFR-B gene. At least four allelic sequences were found to exist by amplification of the DFR-B gene from various sweet potato cultivars. One of these allelic sequences had a 2-kb deletion in the intergenic region and was observed in the cultivars with high anthocyanin content in their storage roots.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alcohol Oxidoreductases / genetics*
  • Alcohol Oxidoreductases / metabolism
  • Base Sequence
  • Cloning, Molecular
  • DNA Transposable Elements
  • Gene Dosage
  • Ipomoea batatas / enzymology
  • Ipomoea batatas / genetics*
  • Ipomoea batatas / metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Polymorphism, Genetic
  • Sequence Analysis, DNA

Substances

  • DNA Transposable Elements
  • Alcohol Oxidoreductases
  • dihydroflavanol 4-reductase

Associated data

  • GENBANK/AB112545
  • GENBANK/AB112668
  • GENBANK/AB112669