Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells

Megumi Hirose; Hitoshi Hashimoto; Norihito Shintani; Megumi Nakanishi; Naohisa Arakawa; Junko Iga; Hitoshi Niwa; Jun-Ichi Miyazaki; Akemichi Baba

doi:10.1016/j.regpep.2004.08.018

Differential expression of mRNAs for PACAP and its receptors during neural differentiation of embryonic stem cells

Regul Pept. 2005 Mar 15;126(1-2):109-13. doi: 10.1016/j.regpep.2004.08.018.

Authors

Megumi Hirose¹, Hitoshi Hashimoto, Norihito Shintani, Megumi Nakanishi, Naohisa Arakawa, Junko Iga, Hitoshi Niwa, Jun-Ichi Miyazaki, Akemichi Baba

Affiliation

¹ Laboratory of Molecular Neuropharmacology, Graduate School of Pharmaceutical Sciences, Osaka University, Suita, Osaka 565-0871, Japan.

PMID: 15620423
DOI: 10.1016/j.regpep.2004.08.018

Abstract

The expressions of mRNAs for pituitary adenylate cyclase-activating polypeptide (PACAP), vasoactive intestinal peptide (VIP), and their receptors (PAC1, VPAC1 and VPAC2) were examined in the five steps of the in vitro neuronal culture model of embryonic stem (ES) cell differentiation. mRNAs for PACAP, VIP, PAC1 receptor, and VPAC2 receptor were moderately expressed in neural stem cell-enriched cultures, while VPAC1 receptor mRNA was most prominently expressed in embryoid bodies (EBs). The expression of PAC1 receptor mRNA was further upregulated after terminal differentiation into neurons. In contrast, the expressions of PAC1 receptor and PACAP mRNAs were markedly decreased after glial differentiation. These results suggest that this in vitro neuronal culture system will be a useful model for future studies on the functional role of the PACAPergic system during different stages of neuronal development.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Differentiation / physiology*
Cells, Cultured
Mice
Multipotent Stem Cells / physiology*
Nerve Growth Factors / biosynthesis*
Nerve Growth Factors / genetics
Nerve Tissue / embryology
Neurons / physiology*
Neuropeptides / biosynthesis*
Neuropeptides / genetics
Neurotransmitter Agents / biosynthesis*
Neurotransmitter Agents / genetics
Pituitary Adenylate Cyclase-Activating Polypeptide
RNA, Messenger / biosynthesis*
RNA, Messenger / genetics
Receptors, Cell Surface / biosynthesis*
Receptors, Cell Surface / genetics
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Receptors, Vasoactive Intestinal Peptide, Type II
Receptors, Vasoactive Intestinal Polypeptide, Type I

Substances

Adcyap1 protein, mouse
Adcyap1r1 protein, mouse
Nerve Growth Factors
Neuropeptides
Neurotransmitter Agents
Pituitary Adenylate Cyclase-Activating Polypeptide
RNA, Messenger
Receptors, Cell Surface
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide
Receptors, Pituitary Adenylate Cyclase-Activating Polypeptide, Type I
Receptors, Vasoactive Intestinal Peptide, Type II
Receptors, Vasoactive Intestinal Polypeptide, Type I
Vipr1 protein, mouse
Vipr2 protein, mouse