Abstract
Erm, a member of the PEA3 group within the Ets family of transcription factors, is expressed in murine and human lymphocytes. Here, we show that in the human Molt4 lymphoblastic cell line, the erm gene expression is regulated by the conventional PKC (cPKC) pathway. To better characterize the molecular mechanism by which cPKC regulates Erm transcription in Molt4 cells, we tested proximal promoter deletions of the human gene, and identified a specific cPKC-regulated region between positions -420 and -115 upstream of the first exon.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line, Tumor
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DNA-Binding Proteins / genetics*
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DNA-Binding Proteins / metabolism
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Dactinomycin / pharmacology
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Gene Expression Regulation / physiology*
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Humans
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Precursor Cell Lymphoblastic Leukemia-Lymphoma
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Promoter Regions, Genetic / genetics*
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Protein Kinase C / antagonists & inhibitors
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Protein Kinase C / physiology*
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RNA, Messenger / analysis
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RNA, Messenger / metabolism
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Sequence Deletion / genetics
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Signal Transduction / physiology*
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T-Lymphocytes / metabolism*
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Tetradecanoylphorbol Acetate / analogs & derivatives*
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Tetradecanoylphorbol Acetate / pharmacology
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Transcription Factors / genetics*
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Transcription Factors / metabolism
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Transcription, Genetic / drug effects
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Transcription, Genetic / physiology
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Up-Regulation
Substances
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DNA-Binding Proteins
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ETV5 protein, human
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RNA, Messenger
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Transcription Factors
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transcription factor PEA3
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Dactinomycin
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phorbolol myristate acetate
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Protein Kinase C
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Tetradecanoylphorbol Acetate