A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis

Masahiko Kuroda; Kosuke Oikawa; Tetsuya Ohbayashi; Keiichi Yoshida; Kazuhiko Yamada; Junsei Mimura; Yoichi Matsuda; Yoshiaki Fujii-Kuriyama; Kiyoshi Mukai

doi:10.1016/j.febslet.2004.11.070

A dioxin sensitive gene, mammalian WAPL, is implicated in spermatogenesis

FEBS Lett. 2005 Jan 3;579(1):167-72. doi: 10.1016/j.febslet.2004.11.070.

Authors

Masahiko Kuroda¹, Kosuke Oikawa, Tetsuya Ohbayashi, Keiichi Yoshida, Kazuhiko Yamada, Junsei Mimura, Yoichi Matsuda, Yoshiaki Fujii-Kuriyama, Kiyoshi Mukai

Affiliation

¹ Department of Pathology, Tokyo Medical University, 6-1-1 Shinjuku, Shinjuku-ku, Tokyo 160-8402, Japan. [email protected]

PMID: 15620708
DOI: 10.1016/j.febslet.2004.11.070

Abstract

2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) is an endocrine disruptor that produces a variety of toxic effects. We have isolated a mouse homolog of the hWAPL gene, termed mouse WAPL (mWAPL), as a target of TCDD by cDNA representational difference analysis from mouse embryonic stem cells. A statistically significant increase in mWAPL expression was observed at 0.1 microM TCDD in AhR-/- mouse embryonic fibroblast cells. Interestingly, at 1 microM TCDD, mWAPL mRNA levels decreased in AhR+/+ cells, but further increased in AhR-/- cells. hWAPL and mWAPL were highly expressed only in testes among normal tissue samples, and we observed mWAPL localization in the synaptonemal complex of testicular chromosomes. In addition, mouse testes decreased the expression of mWAPL mRNA after a single intraperitoneal injection of TCDD. Thus, mammalian WAPL such as hWAPL and mWAPL may be involved in spermatogenesis and be target genes mediating the reproductive toxicity induced by TCDD.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Animals
Cytochrome P-450 CYP1A1 / analysis
Cytochrome P-450 CYP1A1 / genetics
Cytochrome P-450 CYP1A1 / metabolism
Down-Regulation
Fibroblasts / chemistry
Fibroblasts / drug effects
Fibroblasts / metabolism
Gene Expression / drug effects
Gene Expression / genetics
Male
Mice
Molecular Sequence Data
Polychlorinated Dibenzodioxins / toxicity*
Proteins / analysis
Proteins / genetics*
Proteins / physiology*
RNA, Messenger / analysis
RNA, Messenger / metabolism
Receptors, Aryl Hydrocarbon / drug effects
Receptors, Aryl Hydrocarbon / genetics
Receptors, Aryl Hydrocarbon / physiology
Sequence Alignment
Spermatocytes / chemistry
Spermatocytes / drug effects
Spermatocytes / metabolism
Spermatogenesis* / drug effects
Testis / cytology
Testis / drug effects
Testis / metabolism*
Up-Regulation

Substances

Polychlorinated Dibenzodioxins
Proteins
RNA, Messenger
Receptors, Aryl Hydrocarbon
WAPL protein, mouse
Cytochrome P-450 CYP1A1