Interaction of polypyridyl ruthenium (II) complex containing asymmetric ligand with DNA

J Inorg Biochem. 2005 Feb;99(2):530-7. doi: 10.1016/j.jinorgbio.2004.10.030.

Abstract

A novel asymmetric bidentate ruthenium (II) complex, [Ru(bpy)(2)(PYNI)](2+) (bpy=2,2'-bipyridine, PYNI=2-(2'-pyridyl)naphthoimidazole), has been synthesized and characterized by elemental analysis, ES-MS (electrospray mass spectra) and (1)H NMR. The electrochemical behaviors of this complex were studied by cyclic voltammetry. DNA interaction studies suggest that [Ru(bpy)(2)(PYNI)](2+) binds to calf thymus DNA (CT-DNA) in an intercalative mode. Interestingly, this new Ru(II) complex has also been found to promote cleavage of plasmid pBR 322 DNA from the supercoiled form I to the open circular form II upon irradiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cattle
  • DNA / drug effects*
  • In Vitro Techniques
  • Intercalating Agents / chemical synthesis
  • Intercalating Agents / chemistry
  • Intercalating Agents / pharmacology
  • Ligands
  • Luminescence
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Organometallic Compounds / chemical synthesis
  • Organometallic Compounds / chemistry*
  • Organometallic Compounds / pharmacology*
  • Photochemistry
  • Plasmids / drug effects
  • Ruthenium
  • Spectrometry, Mass, Electrospray Ionization
  • Spectrophotometry
  • Thermodynamics
  • Viscosity

Substances

  • Intercalating Agents
  • Ligands
  • Organometallic Compounds
  • Ruthenium
  • DNA