Assessment of blood-induced cartilage damage in rabbit knees using scanning electron microscopy

J Orthop Surg (Hong Kong). 2004 Dec;12(2):199-204. doi: 10.1177/230949900401200212.

Abstract

Purpose: To investigate the harmful effects of a single episode of intra-articular bleeding on articular cartilage of rabbit knees using scanning electron microscopy.

Methods: Autologous blood was injected into the right knee joints of 18 New Zealand white rabbits. Surface and cellular damages were examined by the scanning electron microscope (n=9) and light microscope (n=34), respectively. The injected right knees were then compared with the corresponding control left knees at one, 3, and 6 weeks after the blood injection.

Results: The articular surface of the injected knees turned uniformly rough with multiple pits after one week. Maximal changes with elevations and depressions were observed at 3 weeks. These changes reversed at 6 weeks with the irregularities smoothing out. A similar pattern of transient cartilage damage was noted histologically.

Conclusion: Both scanning electron microscopic and light microscopic findings suggest that a single episode of intra-articular bleeding leads to articular cartilage damage but this appears to be reversible. Our findings of transient damage to the articular cartilage suggest that there is no need for intra-articular evacuation and washout following an acute episode of haemarthrosis.

MeSH terms

  • Animals
  • Cartilage Diseases / etiology*
  • Cartilage Diseases / pathology
  • Cartilage, Articular / pathology*
  • Female
  • Hemarthrosis / complications*
  • Hemarthrosis / pathology
  • Knee Joint*
  • Male
  • Microscopy, Electron, Scanning
  • Rabbits