Morphogenetic and cellular movements that shape the mouse cerebellum; insights from genetic fate mapping

Neuron. 2005 Jan 6;45(1):27-40. doi: 10.1016/j.neuron.2004.12.021.

Abstract

We used the cerebellum as a model to study the morphogenetic and cellular processes underlying the formation of elaborate brain structures from a simple neural tube, using an inducible genetic fate mapping approach in mouse. We demonstrate how a 90 degrees rotation between embryonic days 9 and 12 converts the rostral-caudal axis of dorsal rhombomere 1 into the medial-lateral axis of the wing-like bilateral cerebellar primordium. With the appropriate use of promoters, we marked specific medial-lateral domains of the cerebellar primordium and derived a positional fate map of the murine cerebellum. We show that the adult medial cerebellum is produced by expansion, rather than fusion, of the thin medial primordium. Furthermore, ventricular-derived cells maintain their original medial-lateral coordinates into the adult, whereas rhombic lip-derived granule cells undergo lateral to medial posterior transverse migrations during foliation. Thus, we show that progressive changes in the axes of the cerebellum underlie its genesis.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Body Patterning / genetics
  • Cell Differentiation / genetics*
  • Cell Lineage / genetics*
  • Cell Movement / genetics*
  • Cerebellum / cytology
  • Cerebellum / embryology*
  • Cerebellum / metabolism
  • Chimera
  • Female
  • Gene Expression Regulation, Developmental / genetics
  • Genetic Markers
  • Homeodomain Proteins / genetics
  • Integrases / genetics
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Morphogenesis / physiology*
  • Nerve Tissue Proteins / genetics
  • Neurons / cytology
  • Neurons / metabolism
  • Promoter Regions, Genetic / genetics
  • Stem Cells / cytology
  • Stem Cells / metabolism*

Substances

  • En1 protein, mouse
  • Genetic Markers
  • Homeodomain Proteins
  • Nerve Tissue Proteins
  • engrailed 2 protein
  • Cre recombinase
  • Integrases