Abstract
The tumor necrosis factor family members BAFF and APRIL induce Ig isotype switching in human B cells. We analyzed the ability of BAFF and APRIL to induce isotype switching in murine B cells to IgG1, IgA, and IgE. APRIL and BAFF each engage two receptors, transmembrane activator and calcium-modulator and cytophilin ligand interactor (TACI) and B cell maturation antigen (BCMA), on B cells. In addition, BAFF engages a third receptor on B cells, BAFF-R. To determine the role of these receptors in isotype switching, we examined B cells from mice deficient in TACI, BCMA, and BAFF-R. The results obtained indicate that both TACI and BAFF-R are able to transduce signals that result in isotype switching.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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B-Cell Activating Factor
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B-Cell Activation Factor Receptor
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B-Cell Maturation Antigen
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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Immunoglobulin Class Switching / immunology*
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Immunoglobulin G / metabolism
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Membrane Proteins / metabolism*
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Mice
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Receptors, Tumor Necrosis Factor / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Signal Transduction / immunology*
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Tumor Necrosis Factor Ligand Superfamily Member 13
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Tumor Necrosis Factor-alpha / metabolism*
Substances
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B-Cell Activating Factor
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B-Cell Activation Factor Receptor
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B-Cell Maturation Antigen
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Immunoglobulin G
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Membrane Proteins
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Receptors, Tumor Necrosis Factor
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TNFSF13B protein, human
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Tnfrsf13c protein, mouse
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Tnfrsf17 protein, mouse
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Tnfsf13 protein, mouse
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Tnfsf13b protein, mouse
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Tumor Necrosis Factor Ligand Superfamily Member 13
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Tumor Necrosis Factor-alpha