Phenotypic and molecular analysis of tellurite resistance among enterohemorrhagic Escherichia coli O157:H7 and sorbitol-fermenting O157:NM clinical isolates

J Clin Microbiol. 2005 Jan;43(1):452-4. doi: 10.1128/JCM.43.1.452-454.2005.

Abstract

A total of 66 (98.5%) of 67 enterohemorrhagic Escherichia coli (EHEC) O157:H7 strains had increased potassium tellurite (Te) MICs (32 to 1,024 microg/ml), grew on Te-containing media, and possessed Te resistance (ter) genes, whereas 83 (96.5%) of 86 sorbitol-fermenting (SF) EHEC O157:NM strains had Te MICs of </=4 microg/ml, did not grow on Te-containing media, and lacked ter genes. Optimal detection of SF EHEC O157:NM strains requires Te-independent strategies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Culture Media
  • Drug Resistance, Bacterial / genetics*
  • Escherichia coli Infections / microbiology*
  • Escherichia coli O157 / classification
  • Escherichia coli O157 / drug effects*
  • Escherichia coli O157 / genetics
  • Escherichia coli O157 / metabolism
  • Escherichia coli Proteins / genetics
  • Humans
  • Microbial Sensitivity Tests / methods
  • Phenotype
  • Sorbitol / metabolism*
  • Tellurium / pharmacology*

Substances

  • Culture Media
  • Escherichia coli Proteins
  • Sorbitol
  • potassium tellurate(IV)
  • Tellurium