Abstract
Exposure of mice to a 120 dB SPL broad band click sound for 3 h per day induced expression of hypoxia-inducible factor-1alpha (HIF-1alpha) in the organ of Corti, lateral wall tissue and spiral ganglion cells. In the organ of Corti, HIF-1alpha was expressed in inner and outer hair cells, pillar cells and Deiters' cells, but not in Hensen's cells or the tectorial, Reissner's or basement membrane. HIF-1alpha expression was definitely increased in mice with a permanent threshold shift, where the hearing level did not recover, even after 4 weeks. These results suggest that noise may induce tissue hypoxia in the inner ear and that the further study on the role of HIF-1alpha will be needed.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Acoustic Stimulation / methods
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Animals
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Auditory Threshold / physiology
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Blotting, Western / methods
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DNA-Binding Proteins / metabolism*
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Dose-Response Relationship, Radiation
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Ear, Inner / metabolism
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Ear, Inner / radiation effects*
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Gene Expression Regulation / radiation effects*
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Immunohistochemistry / methods
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Mice
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Mice, Inbred BALB C
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Noise*
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Nuclear Proteins / metabolism*
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Time Factors
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Transcription Factors / metabolism*
Substances
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DNA-Binding Proteins
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Hif1a protein, mouse
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Hypoxia-Inducible Factor 1
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Hypoxia-Inducible Factor 1, alpha Subunit
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Nuclear Proteins
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Transcription Factors