Comparison of a new, affordable flow cytometric method and the manual magnetic bead technique for CD4 T-lymphocyte counting in a northern Nigerian setting

Clin Diagn Lab Immunol. 2005 Jan;12(1):224-7. doi: 10.1128/CDLI.12.1.224-227.2005.

Abstract

We compared two techniques for CD4 T-lymphocyte counting: flow cytometry (Cyflow) and magnetic beads (Dynabead). Similar results with good correlation were obtained from the 40 adult blood samples counted (P=0.057, r=0.93). The Cyflow technique is more precise and cost-effective than the Dynabead method ($3 to $5 versus $12 to $22 per test, respectively), since as many as 200 samples can be measured per day.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Automation
  • CD4 Lymphocyte Count / economics
  • CD4 Lymphocyte Count / methods*
  • Flow Cytometry / economics
  • Flow Cytometry / methods*
  • Humans
  • Immunomagnetic Separation* / economics
  • Immunomagnetic Separation* / methods
  • Nigeria
  • Reproducibility of Results
  • Sensitivity and Specificity