A simple method to study cellular migration

J Neurosci Methods. 2005 Feb 15;141(2):271-6. doi: 10.1016/j.jneumeth.2004.07.001.

Abstract

We describe here a simple and fast method for the characterisation of cell motion. By projecting on a single plane different positions of the cell a ribbon is generated, whose characteristics can be related to the type of motion. The proposed method allows both to determine, very quickly, the motility of a population of cells and to investigate and characterise properties of a single cell's motion. The methodology presented here can be applied to a large range of cell movement and also adapted and extended to other problems involving biological motion.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Cell Movement / drug effects
  • Cell Movement / physiology*
  • Cells, Cultured
  • Chick Embryo
  • Computer Graphics*
  • Computer Simulation
  • Dose-Response Relationship, Drug
  • Fibroblast Growth Factors / pharmacology
  • Glial Cell Line-Derived Neurotrophic Factor
  • Image Processing, Computer-Assisted / methods*
  • Models, Biological
  • Nerve Growth Factors / pharmacology

Substances

  • Glial Cell Line-Derived Neurotrophic Factor
  • Nerve Growth Factors
  • Fibroblast Growth Factors