Abstract
A differential-display PCR procedure identified the capsular assembly gene kpsD after Escherichia coli type 1 fimbrial binding to mannose-coated Sepharose beads. Limiting-dilution reverse-transcribed PCRs confirmed down-regulation of the kpsD gene, and Northern blot and lacZ fusion analyses showed down-regulation of the kpsFEDUCS region 1 operon. KpsD protein levels fell, and an agglutination test showed less K capsular antigen on the surface following the bacterial ligand-receptor interaction. These data show that binding of type 1 fimbriae (pili) to d-mannose receptors triggers a cross talk that leads to down-regulation of the capsule assembly region 1 operon in uropathogenic E. coli.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Bacterial Adhesion / genetics
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Bacterial Adhesion / physiology
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Bacterial Capsules / genetics
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Bacterial Capsules / metabolism*
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Blotting, Northern
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Enzyme-Linked Immunosorbent Assay
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Escherichia coli / genetics
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Escherichia coli / metabolism*
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Escherichia coli Proteins / genetics
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Escherichia coli Proteins / metabolism*
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Fimbriae, Bacterial / metabolism*
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Genes, Reporter
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Lectins, C-Type / metabolism*
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Mannose Receptor
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Mannose-Binding Lectins / metabolism*
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Periplasmic Proteins / genetics
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Periplasmic Proteins / metabolism*
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Promoter Regions, Genetic
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Receptors, Cell Surface / metabolism*
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Time Factors
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Transcription, Genetic / physiology
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Urinary Bladder / microbiology
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Urinary Bladder / physiology
Substances
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Escherichia coli Proteins
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FtsZ84 protein, E coli
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KpsD protein, E coli
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Lectins, C-Type
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Mannose Receptor
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Mannose-Binding Lectins
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Periplasmic Proteins
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Receptors, Cell Surface