A polymorphism exon 1 variant at the locus of the scavenger receptor class B type I (SCARB1) gene is associated with differences in insulin sensitivity in healthy people during the consumption of an olive oil-rich diet

J Clin Endocrinol Metab. 2005 Apr;90(4):2297-300. doi: 10.1210/jc.2004-1489. Epub 2005 Jan 25.

Abstract

Scavenger receptor class B type I (SCARB1) was described as the first high-density lipoprotein receptor. Increasing evidence indicates that SCARB1 plays additional roles particularly in type 2 diabetes mellitus. Our aim was to determine whether the presence of an exon 1 (G-->A) polymorphism at the SCARB1 gene modifies the insulin sensitivity to dietary fat.

Methods: We studied 59 healthy volunteers (30 men and 29 women, 42 G/G homozygous and 17 G/A heterozygous). Subjects consumed three diets for 4 wk each: a saturated fatty acid (SFA)-rich diet (38% fat, 20% SFA), followed by a carbohydrate (CHO)-rich diet (30% fat, 55% CHO) or a monounsaturated fatty acid (MUFA)-rich diet (38% fat, 22% MUFA) after a randomized crossover design. For each diet, we investigated peripheral insulin sensitivity with the insulin suppression test.

Results: Steady-state plasma glucose after the MUFA diet was lower in G/A compared with G/G subjects (P = 0.030). This effect was not observed after CHO and SFA diets (P = 0.177 and 0.957, respectively). Plasma nonesterified free fatty acid values were lower in subjects carrying the A allele for all the diet periods.

Conclusions: Our findings show that carriers of the G/A genotype have significant increases in insulin sensitivity after a MUFA-rich diet compared with G/G individuals.

Publication types

  • Clinical Trial
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Alleles
  • Cross-Over Studies
  • Dietary Carbohydrates / administration & dosage
  • Exons
  • Fatty Acids, Nonesterified / blood
  • Female
  • Humans
  • Insulin Resistance*
  • Male
  • Olive Oil
  • Plant Oils / administration & dosage*
  • Polymorphism, Genetic*
  • Receptors, Immunologic / genetics*
  • Receptors, Immunologic / physiology
  • Receptors, Scavenger
  • Scavenger Receptors, Class B

Substances

  • Dietary Carbohydrates
  • Fatty Acids, Nonesterified
  • Olive Oil
  • Plant Oils
  • Receptors, Immunologic
  • Receptors, Scavenger
  • SCARB1 protein, human
  • Scavenger Receptors, Class B