Abstract
L-Ascorbyl oleate and L-ascorbyl linoleate were synthesized by an immobilized lipase from Candida antarctica with yields of 38% and 44%, respectively. L-Ascorbyl oleate was stable in sterile culture medium over 12 h at 37 degrees C but L-ascorbyl linoleate degraded by 17%. Ascorbyl oleate had a better protective effect on human umbilical cord vein endothelial cells treated with H2O2 than of L-ascorbic acid-2-phosphate-6-palmitate (Asc2P6P).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Antioxidants / chemistry
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Antioxidants / pharmacology*
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Ascorbic Acid / analogs & derivatives*
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Ascorbic Acid / chemical synthesis*
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Ascorbic Acid / chemistry*
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Ascorbic Acid / pharmacology*
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Cell Survival / drug effects
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Cells, Cultured
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Dose-Response Relationship, Drug
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Drug Stability
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Endothelial Cells / cytology*
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Endothelial Cells / drug effects*
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Endothelial Cells / metabolism
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Enzymes, Immobilized / chemistry
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Esterification
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Fungal Proteins
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Humans
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Hydrogen Peroxide / pharmacology*
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Kinetics
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Linoleic Acids / chemical synthesis*
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Linoleic Acids / pharmacology*
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Lipase / chemistry*
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Oleic Acid / chemical synthesis*
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Oleic Acid / pharmacology*
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Oleic Acids*
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Oxidative Stress / drug effects*
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Reactive Oxygen Species / metabolism
Substances
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Antioxidants
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Enzymes, Immobilized
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Fungal Proteins
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Linoleic Acids
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Oleic Acids
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Reactive Oxygen Species
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Oleic Acid
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Hydrogen Peroxide
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Novozyme 435
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Lipase
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Ascorbic Acid
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ascorbyl oleate
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ascorbyl linoleate