Abstract
Ontogenesis and localization expression of genes encoding for the nuclear receptors for oxysterols (lxralpha and lxrbeta) were investigated in human and mouse placenta during gestation. Both mRNAs were detectable in the placentation throughout the gestation (from 7 days postcoitum and 6th week of gestation in mouse and human, respectively) by Northern blots or RT-PCR experiments. Lxralpha showed a higher accumulation in the mouse yolk sac. In situ hybridization pointed a specific expression of lxralpha in the human amniotic membranes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Amnion / embryology
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Amnion / metabolism
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Animals
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Blotting, Northern
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Cell Line
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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Embryonic Development / physiology*
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Female
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Fluorescent Antibody Technique, Indirect
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Gene Expression Regulation, Developmental
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Humans
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In Situ Hybridization
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Liver X Receptors
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Mice
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Orphan Nuclear Receptors
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Placenta / metabolism*
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Pregnancy
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RNA, Messenger / metabolism
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Receptors, Cytoplasmic and Nuclear / genetics
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Receptors, Cytoplasmic and Nuclear / metabolism*
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Reverse Transcriptase Polymerase Chain Reaction
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Yolk Sac / embryology
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Yolk Sac / metabolism
Substances
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DNA-Binding Proteins
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Liver X Receptors
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NR1H3 protein, human
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Nr1h3 protein, mouse
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Orphan Nuclear Receptors
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RNA, Messenger
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Receptors, Cytoplasmic and Nuclear