Use of locked nucleic acid oligonucleotides as hybridization/FRET probes for quantification of 16S rDNA by real-time PCR

Biotechniques. 2005 Jan;38(1):29-30, 32. doi: 10.2144/05381BM01.
No abstract available

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics*
  • Bacteria / isolation & purification
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Fluorescence Resonance Energy Transfer / methods*
  • In Situ Hybridization, Fluorescence / methods*
  • Oligonucleotide Probes / genetics*
  • Oligonucleotides
  • Oligonucleotides, Antisense / genetics*
  • RNA, Ribosomal, 16S / analysis
  • RNA, Ribosomal, 16S / genetics*
  • Reverse Transcriptase Polymerase Chain Reaction / methods*

Substances

  • DNA, Bacterial
  • Oligonucleotide Probes
  • Oligonucleotides
  • Oligonucleotides, Antisense
  • RNA, Ribosomal, 16S
  • locked nucleic acid