Cloning and expression of human lectin-like oxidized low density lipoprotein receptor-1 in Pichia pastoris

Biotechnol Lett. 2005 Jan;27(1):49-52. doi: 10.1007/s10529-004-6582-5.

Abstract

Lectin-like oxidatively-modified LDL receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (oxLDL) in aortic endothelial cells. Human LOX-1 (hLOX-1) gene (cDNA) was cloned from the monocytic leukemic cell line THP-1 and expressed in Pichia pastoris. The recombinant protein (rhLOX-1) was purified by his-tag affinity chromatography. Preliminary identification was performed by Western blot analysis and a ligand-receptor binding assay showed that the protein had specific oxLDL-binding activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Western
  • Cell Line, Tumor
  • Chromatography, Affinity
  • Cloning, Molecular
  • DNA Primers
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Pichia / genetics*
  • Plasmids
  • Receptors, LDL / chemistry
  • Receptors, LDL / genetics*
  • Receptors, Oxidized LDL
  • Recombinant Proteins / chemistry
  • Recombinant Proteins / genetics
  • Scavenger Receptors, Class E

Substances

  • DNA Primers
  • OLR1 protein, human
  • Receptors, LDL
  • Receptors, Oxidized LDL
  • Recombinant Proteins
  • Scavenger Receptors, Class E