Abstract
We developed a duplex PCR assay targeting the hemagglutinin multigene families, vlhA and pMGA, of Mycoplasma synoviae and Mycoplasma gallisepticum, respectively. The assay proved to be specific and sensitive enough to justify its use for the simultaneous detection of the two major avian mycoplasma species from field isolates.
Publication types
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Bacterial Proteins / genetics
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Conserved Sequence
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Hemagglutinins / genetics*
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Mycoplasma Infections / microbiology
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Mycoplasma Infections / veterinary
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Mycoplasma gallisepticum / classification*
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Mycoplasma gallisepticum / genetics
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Mycoplasma gallisepticum / isolation & purification
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Mycoplasma synoviae / classification*
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Mycoplasma synoviae / genetics
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Mycoplasma synoviae / isolation & purification
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Polymerase Chain Reaction / methods*
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Poultry Diseases / microbiology
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Sensitivity and Specificity
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Species Specificity
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Trachea / microbiology
Substances
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Bacterial Proteins
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Hemagglutinins