Abstract
We describe a developmental analysis of Drosophila Cullin-5 (Cul-5) identified from the genome sequence on the basis of its high degree of homology to vertebrate and worm sequences. The gene is expressed in a restricted manner in ectodermal cells throughout development suggesting pleiotropic functions. We decided to examine the phenotypes of Cul-5 aberrations in two well-studied developmental systems: the neuromuscular junction (NMJ) and the developing sensory organ. Alteration of Cul-5 levels in motoneurons results in an increase in bouton number at the NMJ. The cells of a sensory organ on the adult notum arise from a single progenitor cell by regulated cell division. Aberrations in Cul-5 affect different steps in the lineage consistent with a role in cell fate determination, proliferation, and death. Such phenotypes highlight the multiple cellular processes in which Cul-5 can participate.
Copyright (c) 2005 Wiley-Liss, Inc.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Cell Division
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Cullin Proteins / chemistry
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Cullin Proteins / genetics
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Cullin Proteins / metabolism*
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Cullin Proteins / ultrastructure
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Drosophila / embryology*
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Drosophila / genetics
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Drosophila / growth & development
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Drosophila / ultrastructure
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Drosophila Proteins / chemistry
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Drosophila Proteins / genetics
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Drosophila Proteins / metabolism*
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Drosophila Proteins / ultrastructure
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Embryo, Nonmammalian
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Gene Expression
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Gene Expression Regulation
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Genes, Insect
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Larva
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Metamorphosis, Biological
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Molecular Sequence Data
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Mutation
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Neuromuscular Junction / cytology
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Neuromuscular Junction / embryology*
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Neuromuscular Junction / ultrastructure
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Neurons, Afferent / cytology*
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Neurons, Afferent / ultrastructure
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Organogenesis
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Pupa
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RNA, Messenger / metabolism
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Sequence Homology, Amino Acid
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Synapses / metabolism*
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Synapses / ultrastructure
Substances
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Cullin Proteins
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Drosophila Proteins
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RNA, Messenger