Dynamics of anti-M antibody response in a mouse model following intranasal infection with group A Streptococcus M-18

J Med Microbiol. 2005 Mar;54(Pt 3):305-308. doi: 10.1099/jmm.0.45555-0.

Abstract

Dynamics of anti-M antibody response following intranasal infection with group A Streptococcus (GAS) M-18 were investigated in a Swiss albino mouse model. Mice arranged in three groups were inoculated intranasally with 2.0x10(7) c.f.u. ml-1 of GAS M-18 on 1, 2 alternate and 3 alternate days. Plasma collected from the retro-orbital plexus was tested for antibodies by an in-house indirect ELISA. The antibody titres of the plasma samples varied from 1 : 8 to 1 : 1024 in the 1 day dose, from 1 : 4 to 1 : 256 in the 2 day dose and from 1 : 4 to 1 : 128 in the 3 day dose. Peak titres were seen on day 42 or 56 and in all cases the titres had declined by day 84. Swiss albino mouse can thus serve as a useful animal model to study different aspects of type-specific anti-M immune responses against GAS disease when designing candidate streptococcal vaccines.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / biosynthesis*
  • Antibodies, Bacterial / blood
  • Antigens, Bacterial / immunology
  • Disease Models, Animal
  • Enzyme-Linked Immunosorbent Assay
  • Mice
  • Nose / microbiology
  • Streptococcal Infections / immunology*
  • Streptococcus pyogenes / immunology*

Substances

  • Antibodies, Bacterial
  • Antigens, Bacterial