Synthesis of arabinitol 1-phosphate and its use for characterization of arabinitol-phosphate dehydrogenase

Carbohydr Res. 2005 Mar 21;340(4):539-46. doi: 10.1016/j.carres.2004.11.030.

Abstract

D-arabinitol 1-phosphate (Ara-ol1-P), a substrate for D-arabinitol-phosphate dehydrogenase (APDH), was chemically synthesized from D-arabinonic acid in five steps (O-acetylation, chlorination, reduction, phosphorylation, and de-O-acetylation). Ara-ol1-P was used as a substrate for the characterization of APDH from Bacillus halodurans. APDH converts Ara-ol1-P to xylulose 5-phosphate in the oxidative reaction; both NAD(+) and NADP(+) were accepted as co-factors. Kinetic parameters for the oxidative and reductive reactions are consistent with a ternary complex mechanism.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / enzymology
  • Bacillus / genetics
  • Bacillus / metabolism
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / isolation & purification
  • Bacterial Proteins / metabolism
  • Enterococcus / enzymology
  • Enterococcus / genetics
  • Enterococcus / metabolism
  • Kinetics
  • NAD / metabolism
  • NADP / metabolism
  • Oxidation-Reduction
  • Pentosephosphates / chemical synthesis*
  • Pentosephosphates / metabolism*
  • Recombinant Proteins / metabolism
  • Stereoisomerism
  • Substrate Specificity
  • Sugar Alcohol Dehydrogenases / chemistry*
  • Sugar Alcohol Dehydrogenases / genetics
  • Sugar Alcohol Dehydrogenases / isolation & purification
  • Sugar Alcohol Dehydrogenases / metabolism*
  • Xylulose / metabolism

Substances

  • Bacterial Proteins
  • Pentosephosphates
  • Recombinant Proteins
  • NAD
  • NADP
  • Xylulose
  • Sugar Alcohol Dehydrogenases