Abstract
The O-polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O57:H29. Studies by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments, showed that the polysaccharide contains an amide of D-galacturonic acid with L-alanine and has the following pentasaccharide repeating unit: [formula: see text]
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alanine / chemistry*
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Carbohydrate Conformation
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Carbohydrate Sequence
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Hexuronic Acids / chemistry*
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Lipopolysaccharides / chemistry*
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Lipopolysaccharides / isolation & purification
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Nuclear Magnetic Resonance, Biomolecular
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O Antigens / chemistry*
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O Antigens / immunology
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Polysaccharides, Bacterial / chemistry*
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Polysaccharides, Bacterial / isolation & purification
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Providencia / chemistry*
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Providencia / immunology
Substances
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Hexuronic Acids
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Lipopolysaccharides
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O Antigens
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Polysaccharides, Bacterial
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galacturonic acid
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Alanine