The structure of the O-polysaccharide from the lipopolysaccharide of Providencia stuartii O57 containing an amide of D-galacturonic acid with L-alanine

Carbohydr Res. 2005 Mar 21;340(4):775-80. doi: 10.1016/j.carres.2005.01.007.

Abstract

The O-polysaccharide (O-antigen) was obtained by mild acid degradation of the lipopolysaccharide of Providencia stuartii O57:H29. Studies by sugar and methylation analyses along with (1)H and (13)C NMR spectroscopy, including two-dimensional (1)H,(1)H COSY, TOCSY, ROESY, H-detected (1)H,(13)C HSQC, and HMBC experiments, showed that the polysaccharide contains an amide of D-galacturonic acid with L-alanine and has the following pentasaccharide repeating unit: [formula: see text]

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alanine / chemistry*
  • Carbohydrate Conformation
  • Carbohydrate Sequence
  • Hexuronic Acids / chemistry*
  • Lipopolysaccharides / chemistry*
  • Lipopolysaccharides / isolation & purification
  • Nuclear Magnetic Resonance, Biomolecular
  • O Antigens / chemistry*
  • O Antigens / immunology
  • Polysaccharides, Bacterial / chemistry*
  • Polysaccharides, Bacterial / isolation & purification
  • Providencia / chemistry*
  • Providencia / immunology

Substances

  • Hexuronic Acids
  • Lipopolysaccharides
  • O Antigens
  • Polysaccharides, Bacterial
  • galacturonic acid
  • Alanine