Polymerase chain reaction amplification as a diagnostic tool in culture-negative multiple-valve endocarditis

Ann Thorac Surg. 2005 Mar;79(3):e21-2. doi: 10.1016/j.athoracsur.2004.02.082.

Abstract

We report a case of culture-negative infectious endocarditis in a 17-year-old boy in which the etiologic diagnosis could only be provided by polymerase chain reaction amplification and sequencing of the bacterial 16S rRNA gene from valve tissue.

Publication types

  • Case Reports

MeSH terms

  • Adolescent
  • Endocarditis, Bacterial / diagnosis*
  • Endocarditis, Bacterial / microbiology*
  • False Negative Reactions
  • Heart Valve Diseases / diagnosis*
  • Heart Valve Diseases / microbiology*
  • Humans
  • Incidence
  • Male
  • Polymerase Chain Reaction*
  • RNA, Ribosomal, 16S / analysis
  • Streptococcal Infections / diagnosis*
  • Viridans Streptococci / genetics*

Substances

  • RNA, Ribosomal, 16S