A review with 68 references is presented on the application and new developments of CE in pharmaceutical analysis, clinical chemistry and single cell analysis etc. The pharmaceutical analysis includes main component assay, determination of drug-related impurities, stoichiometric determination and quantitative determinations. The Chinese herb analysis includes determinations of main components and prescriptions of Chinese herb. The chiral separation includes separation mechanism and some new chiral selectors. The application of CE in clinical chemistry includes determinations of serum and protein, monitoring of drug metabolism, etc. Single cell analysis includes determinations of component in neurocells and human erythrocyte.