Abstract
An A-B-C type triblock copolymer, tandemly aligning two types of polycations with different pKa values in a single polymer strand, was developed for the construction of novel polyplex micelles, satisfying a high DNA condensing ability as well as a proton buffering activity directed to elevating gene transfection. The micelle might feature the distinctive three-layered structure, where an inner polyplex layer of condensed pDNA with poly(l-lysine) (pKa approximately 9.4) as the C segment is successively wrapped with an intermediate layer of poly[(3-morpholinopropyl)aspartamide] (B segment) with a comparatively low pKa of approximately 6.2, to provide a buffering effect, and an outer PEG layer (A segment) as a biocompatible palisade.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aspartic Acid / administration & dosage
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Aspartic Acid / analogs & derivatives*
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Aspartic Acid / chemistry*
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Buffers
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Cations
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Cell Line
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Gene Transfer Techniques*
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Genetic Vectors / chemical synthesis
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Genetic Vectors / chemistry
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HeLa Cells
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Humans
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Kinetics
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Micelles*
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Morpholines / administration & dosage
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Morpholines / chemistry*
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Plasmids / administration & dosage
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Plasmids / chemistry*
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Plasmids / genetics
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Polyethylene Glycols / administration & dosage
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Polyethylene Glycols / chemistry*
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Polylysine / administration & dosage
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Polylysine / chemistry*
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Transfection
Substances
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Buffers
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Cations
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Micelles
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Morpholines
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poly (3-morpholinopropyl) aspartamide
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Polylysine
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Aspartic Acid
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Polyethylene Glycols