Abstract
Two microarray studies of mediastinal B cell lymphoma have shown that this disease has a distinct gene expression profile, and also that this is closest to the pattern seen in classical Hodgkin's disease. We reported previously an immunohistologic study in which the loss of intracellular B cell-associated signaling molecules in Reed-Sternberg cells was demonstrated, and in this study we have investigated the expression of the same components in more than 60 mediastinal B cell lymphomas. We report that these signaling molecules are frequently present, and in particular that Syk, BLNK and PLC-gamma2 (absent from Reed-Sternberg cells) are present in the majority of mediastinal B cell lymphomas. The overall pattern of B cell signaling molecules in this disease is therefore closer to that of diffuse large B cell lymphoma than to Hodgkin's disease, and is consistent with a common cell of origin as an explanation of the similar gene expression profiles.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptor Proteins, Signal Transducing
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Blotting, Western
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Carrier Proteins / analysis
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Carrier Proteins / biosynthesis*
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DNA-Binding Proteins / analysis
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DNA-Binding Proteins / biosynthesis
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Enzyme Precursors / analysis
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Enzyme Precursors / biosynthesis*
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Hodgkin Disease / metabolism*
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Hodgkin Disease / pathology
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Humans
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Immunohistochemistry
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Intracellular Signaling Peptides and Proteins
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Lymphoma, B-Cell / chemistry
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Lymphoma, B-Cell / metabolism*
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Lymphoma, B-Cell / ultrastructure
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Lymphoma, Large B-Cell, Diffuse / chemistry
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Lymphoma, Large B-Cell, Diffuse / metabolism*
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Lymphoma, Large B-Cell, Diffuse / pathology
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Mediastinal Neoplasms / chemistry
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Mediastinal Neoplasms / metabolism*
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Mediastinal Neoplasms / pathology
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NFATC Transcription Factors
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Nuclear Proteins / analysis
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Nuclear Proteins / biosynthesis
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Phospholipase C gamma
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Phosphoproteins / analysis
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Phosphoproteins / biosynthesis*
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Protein-Tyrosine Kinases / analysis
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Protein-Tyrosine Kinases / biosynthesis*
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Signal Transduction
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Syk Kinase
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Transcription Factors / analysis
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Transcription Factors / biosynthesis
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Type C Phospholipases / analysis
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Type C Phospholipases / biosynthesis*
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src-Family Kinases / analysis
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src-Family Kinases / biosynthesis
Substances
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Adaptor Proteins, Signal Transducing
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B cell linker protein
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Carrier Proteins
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DNA-Binding Proteins
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Enzyme Precursors
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Intracellular Signaling Peptides and Proteins
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NFATC Transcription Factors
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Nuclear Proteins
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Phosphoproteins
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Transcription Factors
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Protein-Tyrosine Kinases
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SYK protein, human
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Syk Kinase
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lyn protein-tyrosine kinase
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src-Family Kinases
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Type C Phospholipases
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Phospholipase C gamma