Abstract
Covert infection with Spodoptera frugiperda multiple nucleopolyhedrovirus, detected by reverse transcription-PCR of virus gene transcripts (ie-0 and polh), was not significantly affected by the presence of an optical brightener (Tinopal UNPA-GX), indicating no change in virus virulence. Detection of the covert infection was dependent on insect life stage and the viral mRNA used for diagnosis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Benzenesulfonates / pharmacology*
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DNA, Viral / genetics
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DNA, Viral / isolation & purification
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Detergents / pharmacology
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Genes, Insect
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Genes, Viral
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Nucleopolyhedroviruses / drug effects*
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Nucleopolyhedroviruses / genetics
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Nucleopolyhedroviruses / pathogenicity
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Reverse Transcriptase Polymerase Chain Reaction
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Spodoptera / growth & development
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Spodoptera / virology*
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Virulence / drug effects
Substances
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Benzenesulfonates
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DNA, Viral
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Detergents
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Tinopal