[Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency]

O F Fedorova; N A Novikova; N V Epifanova; L B Lukovnikova; O N Kniagina; E V Gracheva; E M Bogacheva

[Optimization of RT-PCR for the identification of VP4 gene of group A rotaviruses and evaluation of its diagnostic efficiency]

Vopr Virusol. 2005 Jan-Feb;50(1):39-41.

[Article in Russian]

Authors

O F Fedorova, N A Novikova, N V Epifanova, L B Lukovnikova, O N Kniagina, E V Gracheva, E M Bogacheva

PMID: 15747872

Abstract

A method is suggested for the detection of the human rotavirus VP4 gene by RT-PCR. The method is universal for all [P] types of group A rotavirus in clinical samples. As against RNA electrophoresis and ELISA, it provides for a more accurate detection of rotaviruses by 18.8 and 26.5%, respectively. It was established by RT-PCR in February, 2002, in Nizhny Novgorod, that rotaviruses were responsible for 67% of acute gastroenteritis in hospitalized children.

Publication types

Comparative Study
English Abstract

MeSH terms

Capsid Proteins / analysis
Capsid Proteins / genetics*
Child
DNA Primers
Diagnosis, Differential
Gastroenteritis / diagnosis*
Gastroenteritis / virology
Genome, Viral
Humans
Reverse Transcriptase Polymerase Chain Reaction / methods
Rotavirus / genetics
Rotavirus / isolation & purification*
Rotavirus Infections / diagnosis*
Sensitivity and Specificity

Substances

Capsid Proteins
DNA Primers
VP4 protein, Rotavirus