Relationship between lipocalin-type prostaglandin D synthase and alpha-glucosidase in azoospermia seminal plasma

Clin Chim Acta. 2005 Apr;354(1-2):69-76. doi: 10.1016/j.cccn.2004.11.009. Epub 2004 Dec 30.

Abstract

Background: [corrected] To determine the correlation of lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase in semen.

Methods: We analyzed 68 seminal plasmas for lipocalin-type prostaglandin D synthase (L-PGDS) and alpha-glucosidase, L-PGDS was analyzed by ELISA. The semen donors were categorized in 3 groups: normal, obstructive and non-obstructive azoospermia. We then evaluated their correlation.

Results: The difference of L-PGDS concentration (P<0.001) and alpha-glucosidase activity (P<0.001) among the 3 clinical groups was statistically significant. Correlation between L-PGDS concentration and alpha-glucosidase was also statistically significant. L-PGDS concentration correlated positively with alpha-glucosidase activity (r=0.882).

Conclusions: L-PGDS in seminal plasma, like alpha-glucosidase, suggests an obstruction of the seminal ducts and may be a potential marker that may aid in the differential diagnosis of obstructive and non-obstructive azoospermia.

Publication types

  • Comparative Study

MeSH terms

  • Adult
  • Calibration
  • Enzyme-Linked Immunosorbent Assay
  • Humans
  • Intramolecular Oxidoreductases / analysis*
  • Lipocalins
  • Male
  • Middle Aged
  • Oligospermia / diagnosis
  • Oligospermia / enzymology*
  • Oligospermia / surgery
  • Semen / enzymology*
  • alpha-Glucosidases / analysis*

Substances

  • Lipocalins
  • alpha-Glucosidases
  • Intramolecular Oxidoreductases
  • prostaglandin R2 D-isomerase