Abstract
Demyelinating polyneuropathiy associated with IgM paraproteinemia and high titers of anti-MAG IgM antibodies (MAG-PN) is considered different from chronic inflammatory demyelinating polyneuropathy, particularly because of the poorer response to treatment of MAG-PN patients. Therefore, anti-MAG anitbodies may have relevant prognostic value. Available anti-MAG antibody assays require central nervous system myelin proteins from autopsied human brains. This study investigated the feasibility of detecting anti-MAG antibody by immunofluorescence and flow cytometry using a panel of human neuroblastoma cell lines as targets. We report here on the evaluation of the LA-N-1 cell line as an appropriate substrate for the detection of anti-MAG antibody by indirect immunoflourescence.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Adult
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Aged
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Aged, 80 and over
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Antibodies, Anti-Idiotypic / immunology
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Antibodies, Anti-Idiotypic / metabolism*
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Binding, Competitive / immunology
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CD57 Antigens / immunology
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CD57 Antigens / metabolism
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Cell Line, Tumor
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Demography
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Enzyme-Linked Immunosorbent Assay / methods
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Female
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Flow Cytometry / methods
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Humans
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Immunoblotting / methods
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Male
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Middle Aged
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Myelin-Associated Glycoprotein / immunology*
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Myelin-Associated Glycoprotein / metabolism
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Neuroblastoma / immunology
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Neuroblastoma / metabolism*
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Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / blood
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Polyradiculoneuropathy, Chronic Inflammatory Demyelinating / immunology*
Substances
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Antibodies, Anti-Idiotypic
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CD57 Antigens
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Myelin-Associated Glycoprotein
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anti-IgM