Polymorphisms in SPINK5 are not associated with asthma in a Dutch population

J Allergy Clin Immunol. 2005 Mar;115(3):486-92. doi: 10.1016/j.jaci.2004.12.013.

Abstract

Background: Asthma and allergic phenotypes are complex genetic diseases with known linkage to chromosome 5q. This region has many candidate genes, including serine protease inhibitor Kazal type 5 (SPINK5), which has been associated with asthma and atopic dermatitis in family-based studies of children with atopic dermatitis.

Objective: We sought to investigate whether single nucleotide polymorphisms in SPINK5 are associated with asthma, atopic phenotypes, and atopic dermatitis.

Methods: We investigated whether single nucleotide polymorphisms in SPINK5 (ie, -785 A/G, Asn368Ser, and Lys420Glu) are associated with asthma, atopic phenotypes, and atopic dermatitis in 200 families ascertained by a proband with asthma (nonaffected spouses served as a matched control population) and an independent set of 252 trios with asthma.

Results: We found no association with asthma, atopic phenotypes, and atopic dermatitis after correction for multiple testing.

Conclusion: The negative results in this study suggest that SPINK5 is not associated with asthma or atopic phenotypes in individuals ascertained by a proband with asthma. This is consistent with the finding that SPINK5 is not expressed in the lung. Because our patients were ascertained for asthma, a role of SPINK5 in atopic dermatitis cannot be excluded.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Asthma / genetics*
  • Carrier Proteins / genetics*
  • Dermatitis, Atopic / genetics
  • Female
  • Gene Frequency
  • Humans
  • Male
  • Middle Aged
  • Netherlands
  • Polymerase Chain Reaction
  • Polymorphism, Single Nucleotide / genetics*
  • Proteinase Inhibitory Proteins, Secretory
  • Serine Peptidase Inhibitor Kazal-Type 5

Substances

  • Carrier Proteins
  • Proteinase Inhibitory Proteins, Secretory
  • SPINK5 protein, human
  • Serine Peptidase Inhibitor Kazal-Type 5