(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion

Ivano Bertini; Isabella C Felli; Rainer Kümmerle; Claudio Luchinat; Roberta Pierattelli

doi:10.1007/s10858-005-1679-2

(13)C-(13)C NOESY: a constructive use of (13)C-(13)C spin-diffusion

J Biomol NMR. 2004 Nov;30(3):245-51. doi: 10.1007/s10858-005-1679-2.

Authors

Ivano Bertini¹, Isabella C Felli, Rainer Kümmerle, Claudio Luchinat, Roberta Pierattelli

Affiliation

¹ Magnetic Resonance Center and Department of Chemistry, University of Florence, Italy. [email protected]

PMID: 15756458
DOI: 10.1007/s10858-005-1679-2

Abstract

(13)C-(13)C NOESY experiments were performed under long mixing time conditions on reduced human superoxide dismutase (32 kDa, (15)N, (13C) and 70% (2)H labeled). (13)C-(13)C couplings were successfully eliminated through post-processing of in-phase-anti-phase (IPAP) data. It appears that at mixing time tau(m) of 3.0 s the spin diffusion mechanism allows the detection of 96% of the two-bond correlations involving C' and C(beta). The interpretation was confirmed by simulations. This approach broadens the range of applicability of (13)C-(13)C NOESY spectroscopy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms*
Carbon Isotopes / chemistry
Diffusion
Humans
Nitrogen Isotopes / chemistry
Nuclear Magnetic Resonance, Biomolecular / methods*
Spin Labels*
Superoxide Dismutase / chemistry*

Substances

Carbon Isotopes
Nitrogen Isotopes
Spin Labels
Superoxide Dismutase