Increased expression of matrix metalloproteinase-2 in nasal polyps

Acta Otolaryngol. 2004 Dec;124(10):1165-70. doi: 10.1080/00016480410017152.

Abstract

Objective: The formation and growth of nasal polyps requires remodeling of the extracellular matrix. Matrix metalloproteinases (MMPs) are a family of endopeptidases that can collectively degrade almost all extracellular matrix components. The aim of this study was to investigate the role of MMPs in nasal polyps.

Material and methods: The expression of MMP-2 and -9 was investigated in the nasal polyps of 20 patients undergoing endonasal sinus surgery and compared with that in control nasal mucosal samples obtained from 20 non-allergic hypertrophic rhinitis patients. Tissue samples were either homogenized for reverse transcriptase polymerase chain reaction analysis or frozen for immunohistochemistry.

Results: MMP-2 mRNA expression was observed only in the nasal polyp samples and not in the control tissues. MMP-9 mRNA was not expressed in either type of tissue. Similarly, MMP-2 expression was observed in the nasal polyps of chronic sinusitis patients but not in the inferior turbinate mucosa of the control subjects. MMP-2 positivity was present in basal cells in the surface epithelium and in infiltrating cells in subepithelial tissues. MMP-9-positive cells were not observed in either nasal polyps or inferior turbinates. These results suggest that MMP-2 expression is characteristic of nasal polyps.

Conclusion: MMP-2 may play a role in the remodeling of nasal polyps.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism
  • Adult
  • Aged
  • Chronic Disease
  • Female
  • Humans
  • Immunohistochemistry
  • Male
  • Matrix Metalloproteinase 2 / metabolism*
  • Matrix Metalloproteinase 9 / metabolism
  • Middle Aged
  • Nasal Mucosa / enzymology
  • Nasal Polyps / complications
  • Nasal Polyps / enzymology*
  • Nasal Polyps / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sinusitis / complications
  • Sinusitis / enzymology
  • Turbinates

Substances

  • Actins
  • Matrix Metalloproteinase 2
  • Matrix Metalloproteinase 9