Structure determination of bioactive galloyl derivatives by NMR spectroscopy

Magn Reson Chem. 2005 Jun;43(6):486-8. doi: 10.1002/mrc.1571.

Abstract

The investigation of the chemical constituents of Symplocos racemosa Roxb led to the isolation of two new glycosides, symcomoside A (1) and symcomoside B (2), together with one known glycoside, tortoside C (3), which is reported for the first time from this plant. The structures of the new compounds were determined by 1D and 2D homonuclear and heteronuclear NMR spectroscopy, from chemical evidence and by comparison with published data for closely related compounds. Symcomoside B (2) showed potent inhibitory activity against alpha-glucosidase in a concentration-dependent fashion with an IC50 value of 0.733 +/- 0.033 mM whereas symcomoside A (1) showed very weak inhibitory activity against alpha-glucosidase (9.90% in 0.70 mM).

MeSH terms

  • Carbon Isotopes
  • Gallic Acid / analogs & derivatives*
  • Gallic Acid / chemistry*
  • Gallic Acid / isolation & purification
  • Glucosides / chemistry*
  • Glucosides / isolation & purification
  • Glycosides / chemistry*
  • Glycosides / isolation & purification
  • Magnetic Resonance Spectroscopy / methods*
  • Magnetic Resonance Spectroscopy / standards*
  • Magnoliopsida / chemistry*
  • Molecular Conformation
  • Plant Extracts / chemistry*
  • Plant Extracts / isolation & purification
  • Protons
  • Reference Standards

Substances

  • Carbon Isotopes
  • Glucosides
  • Glycosides
  • Plant Extracts
  • Protons
  • symcomoside A
  • symcomoside B
  • Gallic Acid