An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast

Takayuki Motoyama; Tomohiro Ohira; Kaori Kadokura; Akihiko Ichiishi; Makoto Fujimura; Isamu Yamaguchi; Toshiaki Kudo

doi:10.1007/s00294-005-0572-6

An Os-1 family histidine kinase from a filamentous fungus confers fungicide-sensitivity to yeast

Curr Genet. 2005 May;47(5):298-306. doi: 10.1007/s00294-005-0572-6. Epub 2005 Mar 18.

Authors

Takayuki Motoyama¹, Tomohiro Ohira, Kaori Kadokura, Akihiko Ichiishi, Makoto Fujimura, Isamu Yamaguchi, Toshiaki Kudo

Affiliation

¹ RIKEN (Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan. [email protected]

PMID: 15776234
DOI: 10.1007/s00294-005-0572-6

Abstract

Three groups of fungicides (phenylpyrroles, dicarboximides, aromatic hydrocarbons) are effective against filamentous fungi. The target of these fungicides is the osmotic stress signal transduction pathway, which is dependent on the Os-1 family of two-component histidine kinases. These fungicides usually have no fungicidal effect on the yeast Saccharomyces cerevisiae. In this report, we found that expression of Hik1, an Os-1 orthologue from rice blast fungus, can confer fungicide-sensitivity to yeast. This requires both the histidine kinase and the response regulator domains of Hik1. Analysis of yeast mutants indicated that this sensitivity is Hog1- and Ssk1-dependent. In addition, our studies revealed an interaction between Hik1 and Ypd1. These observations suggest that Hik1 is a direct target of the fungicides or is a mediator of fungicide action and that the fungicidal effect is transmitted to the Hog1 pathway via Ypd1.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Base Sequence
DNA-Binding Proteins / drug effects
DNA-Binding Proteins / genetics
DNA-Binding Proteins / metabolism
Drug Resistance, Fungal / genetics*
Fungicides, Industrial / pharmacology*
Histidine Kinase
Intracellular Signaling Peptides and Proteins
MAP Kinase Signaling System / drug effects
MAP Kinase Signaling System / genetics
Magnaporthe / enzymology*
Magnaporthe / genetics
Mitogen-Activated Protein Kinases / drug effects
Mitogen-Activated Protein Kinases / genetics
Mitogen-Activated Protein Kinases / metabolism
Molecular Sequence Data
Protein Kinases / drug effects
Protein Kinases / genetics*
Protein Kinases / metabolism
Saccharomyces cerevisiae / drug effects*
Saccharomyces cerevisiae / genetics
Saccharomyces cerevisiae Proteins / drug effects
Saccharomyces cerevisiae Proteins / genetics
Saccharomyces cerevisiae Proteins / metabolism

Substances

DNA-Binding Proteins
Fungicides, Industrial
Intracellular Signaling Peptides and Proteins
Saccharomyces cerevisiae Proteins
Protein Kinases
YPD1 protein, S cerevisiae
HOG1 protein, S cerevisiae
Mitogen-Activated Protein Kinases
Histidine Kinase